Conditioned medium and secretome from epididymal epithelial cell cultures improve sperm kinetics and capacitation

Background and Aim: Sperm maturation occurs in the epididymis through interactions with existing molecules inside lumen. However, mechanism of molecular transfer is currently unclear. This study was aimed to determine necessity epididymal epithelial cells (EECs) process sperm terms kinetics tyrosine phosphorylation. Materials Methods: A true experimental research design used this study. The medium tested a primary culture mice caput (cells medium), conditioned (CM) (supernatant EECs), secretome (CM filtered at 0.22 µm). cocultured EEC culture, CM, for 1, 2, 3, or 4 h. original as control. kinetic analysis performed after indicated times using computer-assisted analysis, phosphorylation detected Western blot technique. Results: EECs successfully generated. results showed increased motility progressive movement 3 h incubation (p < 0.05). There significant decrease average path velocity (VAP) values 0.05), but there no change groups. culture-CM groups progressivity VAP percentage compared control In motility, CM were significantly different from medium, group not. Conclusion: cultured secretome, incubation, suggesting that can be replace EECs, especially when analyzing secreted by epithelium during maturation. highlight potential therapy mediums abnormalities. Keywords: kinetics,